In the Journal of Biomaterials and Tissue Engineering, Hedvika Davis, et al, used UCT Specialties silane T2910 (diethylenetriamine trimethoxysilylpropyldiethylenetriamine) in their in vitro CNS myelination model (J. Biomater. Tissue Eng. 2, 206-215, 2012). The T2910, or DETA, modifies the surface of a glass coverslip. Motorneurons are then attached to the coverslip via the T2910. The motorneurons, in turn, were seeded with OPCs (oligiodendrocyte precursor cells) and then allowed to culture. The DETA used gave the surface the following advantages:
·The tramine moiety of T2910 resembles the structure of spermidine, a well known growth factor
·T2910 is an aminosilane that forms a covalently bound, uniform, self-assembled monolayer on glass surfaces
·At physiologic pH, the amines in T2910 carry partial positive charges, providing a hydrophilic surface that promotes cellular attachment
·T2910 also promotes long-term cell survival because it is non-digestible by matrix proteases secreted by cells.
·By using T2910 micropatterning of the culture can now be performed using photolithorgraphy. This cannot be performed using current attachment techniques.
UCT Specialties (Petrarch) continues to manufacture high quality specialty silanes, silicones, catalysts and coatings for a vast variety of manufacturing markets. Coupled with our excellence in the Solid Phase Extraction chromatography markets, this demonstrates UCT Inc. as a leader for your silicon related chemistry products.